The following equipment was purchased for the GSD Ib research. The purchasing price is listed on each item is specially discounted prices for GSD Laboratory after negotiation. Regular retail prices would be very much higher.
1. SpectraMax i3x Multi-Mode Detection Platform, Molecular Devices Inc.: $42,970.51
We are using this multi-functional machine for DNA analysis (concentration and purity for genotyping and gene therapy vector preparation), blood metabolites analysis (measure blood triglyceride, lactate, glucose, uric acid, cytokines etc.), protein analysis (measure specific protein expression) and cellular function analysis (neutrophil function).
2. CFX96 Touch™ Real-Time PCR Detection System, Bio-Rad Life science product: $21,000
This system is used for gene expression detection, DNA copy number calculation, and cDNA synthesis. With this machine, we can analyze various target gene expression in the GSD-Ib knock out cells and mouse/human tissues. Finding significant changes in gene expression could be applied to develop new treatment for the disease.
3. Refrigerated- Micro 21 Microcentrifuge Thermo Scientific™: $3,646.80
In molecular biology, centrifuges are very common laboratory equipment to purify and separate samples such as DNA and RNA. This refrigerated centrifuge is essential equipment for handling temperature sensitive fragile RNA and protein (related to gene expression analysis). It is also used for other temperature sensitive reagents.
4. Cytospin™ 4 Cytocentrifuge, Thermo Fisher Scientific: $8,680
This special centrifuge has designed for making the cells visible. To see the cell structure and shape, cells have to be attached to slide glass and stained with special dyes. Immune cells such as neutrophils have same characteristics as other cells. Therefore, we need to make the cell attached to the slide glass first to see under microscope. This equipment is being used for GSD-Ib HL60 cell line (neutrophil cell line) generation study and will be used in hematopoietic stem cell researches in the future.
5. ZOE Fluorescent Cell Imager, Bio-Rad Life science product: $9,000
This new generation microscope allows our team to see gene expression (protein expression) on the cells using fluorescent dyes (green, red, yellow or blue). Images can be seen and pictures taken to permanently store the images. This equipment is being used in the GSD-Ib HL60 cell line (neutrophil cell line) generation study.
6. -86°C Low Temperature Freezer, Fisher Science: $10,050 each for a total of $20,100
Research samples such as tissue, serum/plasma, DNA, RNA, protein and expensive reagents cannot be stored in regular freezer. This extremely low temperature maintaining freezer enables to store those samples or reagents without degradation.
7. Enduro GDS (Gel Documentation System), Denville Scientific: $9024.07
DNA is invisible until stained by special dyes such as Ethidium Bromide or SYBR green. This equipment uses UV light to visualize the stained DNA and analyze the size of the DNA. This is essential machine for molecular biology. We utilize it in genotyping and DNA sequencing researches.